NovaF– is a K-12 strain suitable for general cloning and plasmid DNA preparation. A derivative of NovaBlue lacking the F factor, NovaF- is the preferred host for cloning in pETcoco™ vectors, which carry the F episome oriS replicon, and the Gateway® Nova destination vectors. NovaF– Singles™ Competent Cells feature high transformation efficiencies and mutations in endA and recA for high yields of high-quality plasmid DNA. Unlike NovaBlue, NovaF– does not allow blue/white screening by alpha complementation and does not provide a source of lacIq repressor.
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Irritant |
||
| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 22-26-36 Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
||
| R Phrase |
R: 36/38 |
||
