For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
04 February 2012
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This product has been discontinued.
We are offering Anti-Cytochrome c Mouse mAb (6H2.B4) (Cat. No. AP1030) or Anti-Cytochrome c (Ab-1) Sheep pAb (Cat. No. PC323)as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
Recognizes the ~15 kDa cytochrome c protein in MCF-7, P388D1, Hela, Jurkat, or NIH/3T3 cells.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG2b | ||
| Immunogen | equine cytochrome c | ||
| Clone | 7H8.2C12 | ||
| Form | Liquid | ||
| Formulation | In 50 mM sodium phosphate. | ||
| Species reactivity | avian, horse, human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
|
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| Positive control | MCF7, P388D1, Hela, Jurkat, or NIH/3T3 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Irritant |
||
| Data | |||
|---|---|---|---|
 Lane 1: Detection of human cytochrome <i>c</i> by immunoblotting. Samples: Whole cell lysate (50 μg) from MCF7 cells. Primary antibodies: Anti-Cytochrome <i>c</i> Mouse mAb (7H8.2C12) (Cat. No. AP1029) (1 μg/ml). Detection: chemiluminescence. Lanes 2,3: Detection of human cytochrome <i>c</i> by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 μg) from MCF7 cells. Antibody for immunoprecipitation: Anti-Cytochrome <i>c</i> Mouse mAb (7H8.2C12) (Cat. No. AP1029) (10 μg/ml) (lane 2) and Anti-Cytochrome <i>c</i> Mouse mAb (6H2.B4) (Cat. No. AP1030) (10 μg/ml) (lane 3). Immunoblotting conditions: same as lane 1.  Detection of human cytochrome <i>c</i> by immunocytochemistry. Sample: HeLa cells fixed with 100% methanol. Primary antibody: Anti-Cytochrome <i>c</i> Mouse mAb (7H8.2C12) (Cat. No. AP1029) (3 μg/ml). Detection: fluorescence with DAPI counterstain. |
