Recognizes the ~75 kD sphingosine kinase 2 protein in transfected HEK293 cells and breast and hepatic carcinoma tissue.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide corresponding to amino acids near the C-terminus of human SPHK2, conjugated to KLH | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human | ||
| Preservative | ≤ 0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Breast carcinoma | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human sphingosine kinase 2 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with SphK2 expressing a c-Myc tag. Primary antibodies: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054) (lane 1) and antibody specific for the c-Myc tag (lane 2). Detection: chemiluminescence.  Detection of human sphingosine kinase 2 by staining paraffin sections. Samples: human breast carcinoma tissue. Primary antibody: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054). Detection: AEC. |
