Detects Akt when phosphorylated on Thr308. Akt is activated by a variety of stimuli including insulin through phosphorylation on Thr308 and Ser473. Once phosphorylated Akt migrates to the nucleus where it is involved in a variety of cellular processes such as the transduction of signals that prevent apoptosis. Although this kit was developed for human samples, it has been found to cross react with mouse and rat.
| Accessories for PhosphoDetect™ Akt (pThr³08) ELISA Kit |
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Product information
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Format
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96-well plate
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Form
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96 Tests
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Detection method
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Colorimetric
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Species reactivity
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human, mouse, rat
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Avoid freeze/thaw
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Yes
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Sensitivity
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≤0.8 units/ml
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Assay range
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1.6-100 units/ml
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Assay time
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4 h
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Sample type
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Cell lysates
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Positive control
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Akt (pThr³08)
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Kit contains
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Coated 96-Well Plate, Akt Phospho-Thr³08 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol.
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Store and ship information
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Storage
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+2°C to +8°C
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Ship
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Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS
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Canadian export regulations
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Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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Data
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The data presented in figure 1 shows that the sensitivity of the ELISA is approximately the same as that of immunoblotting. The bands shown in the immunoblot were developed using Anti-Akt, Phospho-Specific (Thr<sup>308</sup>), (Cat. No. 124001), an alkaline phosphatase conjugated anti-rabbit IgG analyzed by chemiluminescent substrate, and autoradiography.
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