Detects p53 when phosphorylated on Ser15 in human cell lysates. Does not react with mouse or rat. p53 is phosphorylated on Ser15 by ATM, ATR, and DNA protein kinase leading to reduced interactions with its negative regulator, MDM2, and accumulation of p53.
| Accessories for PhosphoDetect™ p53 (pSer¹5) ELISA Kit |
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Product information
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Format
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96-well plate
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Form
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96 Tests
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Detection method
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Colorimetric
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Species reactivity
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human
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Avoid freeze/thaw
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Yes
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Unit definition
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One unit is defined as the amount of p53 (pSer¹5) derived from approximately 11,000 CCRF/CEM cells treated with 5 nM actinomycin D for 20 hours.
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Sensitivity
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≤0.9 units/ml
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Assay range
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1-100 units/ml
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Assay time
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4 h
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Sample type
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Cell lysates
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Kit contains
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Coated 96-Well Plate, p53 Phospho-Ser¹5 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol.
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Store and ship information
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Storage
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+2°C to +8°C
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Ship
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Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS
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