Detects and quantifies the levels of insulin receptor (IR) that are phosphorylated at Tyr1162/1163. The IR is a cell surface receptor that belongs to the superfamily of the growth factor receptor tyrosine kinases and regulates multiple signaling pathways. It is activated by two separate events, where three residues, Tyr1158, Tyr1162 and Tyr1163, located in the regulatory loop, appear to play a major role. Although this kit is designed for use with human cell lines, platelets and lymphocytes, it cross-reacts with mouse and rat cells.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Unit definition | One unit is defined as the amount of IR (pTyr1162/1163) derived from 0.6 ng of IR (β-subunit) in CHO-T cells stimulated with 100 nM insulin. | ||
| Sensitivity | <0.8 Units/ml | ||
| Assay range | 1.6-100 Units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Positive control | Human Insulin Receptor (β-subunit) | ||
| Kit contains | IR (pTyr1162/1163) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using cell lysate with known quantities IR (pTyr<Sup>1162/1163</sup>). The data presented in Figure 1 show that the sensitivity of the ELISA is about 2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using rabbit anti- IR (pTyr<sup>1162/1163</sup>), and chemiluminescent detection. |
