For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
07 February 2012
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Detects and quantifies the level of IκBα independent of its phosphorylation state. IκBα belongs to IκB protein family and is known to modulate NF-kB (nuclear factor-κB) nuclear translocation. It plays an important role in immune and inflammatory responses, cell division and apoptosis.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, not mouse, not rat | ||
| Sensitivity | < 30 pg/ml | ||
| Assay range | 62.5-4000 pg/ml | ||
| Assay time | 4 h | ||
| Kit contains | IκBα Standard, Standard Diluent Buffer, IκBα Antibody-Coated 96-Well Plate, Rabbit Anti-IκBα Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 Known quantities of IκBα protein were measured as outlined in the Detailed Protocol and by immunoblotting using an anti-IκBα antibody and chemiluminescent detection. The ELISA is approximately 2X more sensitive than immunoblotting. |
