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EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: emdinfo@emdchemicals.com
03 September 2010
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Detects and quantifies the level of MEK1 protein independent of its phosphorylation state. MEK1 is a dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as ERK 1 and 2. It is involved in a diverse array of cellular processes such as stress activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Sensitivity | < 0.1 ng/ml | ||
| Assay range | 0.16-10 ng/ml | ||
| Assay time | 4 h | ||
| Kit contains | MEK1 Standard, Standard Diluent Buffer, MEK1 Antibody-Coated 96-Well Plate, Rabbit Anti-MEK1 Detector Antibody, Goat Anti-Rabbit IgG-HRP Concentrate, Sample Treatment Buffer, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS |
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| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of MEK1. The data show that the sensitivity of the ELISA is approximately 2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using a rabbit anti-MEK1 antibody and chemiluminescent detection.  Jurkat cells were grown in tissue culture medium containing 10% fetal calf serum and lysed with Cell Lyis Buffer. This lysate was pre-treated and then diluted in Standard Diluent Buffer over the range of the assay and measured for MEK1. The neat dilution corresponds to a 1:10 final, in-well dilution of cell lysate. Linear regression analysis of sample values versus the expected concentration yielded a correlation coefficient of 0.99.  The data show that the kit detects MEK1 in human cell lysates HT1080, MCF7, and Jurkat, mouse cell lysate NIH3T3, and rat cell lysate Y3. The levels of MEK1 protein detected with this ELISA kit are consistent with results obtained by immunoblot analysis (see inset). |
