Recognizes the ~62-66 kDa Chk2 protein phosphorylated at Thr68 in UV- or doxorubicin-treated HEK293 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Thr68 phosphorylation site of human Chk2 | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | ||
| Species reactivity | human, monkey | ||
| Preservative | None | ||
| Applications |
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| Positive control | HEK 293 cells treated with UV or doxorubicin | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human Chk2, phosphorylated at Thr<sup>68</sup>, by immunoblotting. Samples: Whole cell lysate from HEK293 cells, left untreated (lane 1), treated with UV (lane 2), or treated with 0.5 µM doxorubicin (Cat. No. 324380) (lane 3). Primary antibody: PhosphoDetect™ Anti-Chk2 (pThr<sup>68</sup>) Rabbit pAb (Cat. No. DR1026). Detection: chemiluminescence. |
