Recognizes the ~95 kDa NBS1 protein in HeLa cell nuclear extract.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide corresponding to amino acids encoded by exons 12 and 13 of human NBS1 | ||
| Form | Liquid | ||
| Formulation | In Tris-citrate/phosphate buffer, pH 7.0-8.0. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | HEK293T cells or HeLa cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Highly Toxic |
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| Data | |||
|---|---|---|---|
 Left Lane; IB: Detection of human NBS1 by immunoblotting. Sample: Nuclear extract (50 µg) from HeLa cells. Primary antibody: Anti-NBS1 Rabbit pAb (Cat. No. DR1033) (0.08 µg/ml). Detection: chemiluminescence. Right Lane; IP/IB: Detection of human NBS1 by immunoprecipitation followed by immunoblotting. Sample: Nuclear extract (1 mg) from HeLa cells. Antibody for IP: Anti-WRN (400-450) Rabbit pAb (Cat. No. DR1033) (1.25 µg/mg protein). Immunoblotting conditions: same as left lane.  Left Lane; IB: Detection of human NBS1 by immunoblotting. Sample: Nuclear extract (50 µg) from HeLa cells. Primary antibody: Anti-NBS1 Rabbit pAb (Cat. No. DR1033) (0.08 µg/ml). Detection: chemiluminescence. Right Lane; IP/IB: Detection of human NBS1 by immunoprecipitation followed by immunoblotting. Sample: Nuclear extract (1 mg) from HeLa cells. Antibody for IP: Anti-WRN (400-450) Rabbit pAb (Cat. No. DR1033) (1.25 µg/mg protein). Immunoblotting conditions: same as left lane. |
