Recombinant, rat glial derived neurotrophic factor expressed in S. frugiperda insect cells. Native GDNF exists as a disulfide homodimeric glycoprotein and is a novel member of the TGF-β superfamily. Titrate the protein for optimal results in individual systems. Immbolized rrGFRa/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml.
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Product information
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Form
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Lyophilized
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Formulation
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Lyophilized from 35% Acetonitrile, 0.1% TFA, 50 µg BSA/µg GDNF.
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Avoid freeze/thaw
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Yes
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Biological activity
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ED50 = 1-3 ng/ml as measured by the ability to support the survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia.
Immbolized rrGFRα/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml.
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Purity
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≥97% by SDS-PAGE
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Contaminants
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Endotoxin: ≤1 EU/µg GDNF
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Solubility
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Sterile PBS containing ≥0.1% HSA or BSA
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Store and ship information
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Storage
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-20°C
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Ship
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Dry Ice Only
Standard Handling
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Canadian export regulations
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Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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