PK1006
PhosphoDetect™ Anti-c-Abl (pThr7³5) Rabbit pAb
PhosphoDetect™ Anti-p150 (pThr7³5) Rabbit pAb, PhosphoDetect™ Anti-Abl1 (pThr7³5) Rabbit pAb, Anti-p150, Phospho-Specific (Thr7³5) Rabbit pAb, Anti-Abl1 Phospho-Specific (Thr7³5) Rabbit pAb
Recognizes the ~145 kDa c-Abl and the ~190 kDa and ~210 kDa forms of Bcr-Abl phosphorylated at Thr735 in sorbitol-treated HeLa cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Thr7³5 phosphorylation site of human c-Abl, coupled to KLH | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | ||
| Species reactivity | human | ||
| Preservative | None | ||
| Applications |
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| Positive control | Sorbitol-treated HeLa cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human c-Abl, phosphorylated on Thr<sup>735</sup>, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with sorbitol (0.5 M, 30 min) or left untreated. Primary antibody: PhosphoDetect™ Anti-c-Abl (pThr<sup>735</sup>) Rabbit pAb (Cat. No. PK1006) (1:1000) (upper panel) or total c-Abl antibody (lower panel). Detection: chemiluminescence. |
