QIA21
FragEL™ DNA Fragmentation Detection Kit, Colorimetric - Klenow Enzyme
TUNEL Assay
For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: emdinfo@emdchemicals.com
03 September 2010
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Non-isotopic kit for the detection of apoptosis at the individual cell level. Apoptotic morphology is easily detected. This kit allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, or in cell preparations fixed on slide by Fragment End Labeling (FragEL™) of DNA. Klenow binds to exposed ends of DNA fragments generated in response to apoptotic signals and catalyzes the template-dependent addition of biotin-labeled and unlabeled deoxynucleotides. Biotinylated nucleotides are detected using streptavidin-horseradish peroxidase (HRP) conjugate. Diaminobenzidine reacts with the labeled sample to generate an insoluble colored product at the site of DNA fragmentation. Counterstaining with methyl green aids in the morphological evaluation and characterization of normal and apoptotic cells.
| Product information | |||
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| Format | Microscopy | ||
| Form | 50 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | a broad range of species | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Assay time | 4 h | ||
| Sample type | Frozen or paraffin sections or fixed-cell preparations | ||
| Kit contains | Counterstain, Proteinase K, Equilibration and Labeling Buffers, Klenow Enzyme (Cat. No. PF059), Stop buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H2O2/Urea Tablets, Methyl Green Counterstain, and a user protocol. | ||
| Store and ship information | |||
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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