A quantitative colorimetric assay for the measurement of Bcl-2. Able to detect significant decreases in Bcl-2 protein levels before significant levels of apoptosis are detected. Kit has high sensitivity, a broad dynamic range, and contains all required buffers.
One unit of Bcl-2 equals the Bcl-2 activity in 5.6 X 104 HL-60 cells
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Sensitivity | < 1 ng/ml | ||
| Assay time | 3.5 h | ||
| Sample type | Cell lysates, tissue culture supernatant, sera, or plasma | ||
| Kit contains | 96 Well Coated Plate, Antigen Extraction Reagent, Lyophilized Bcl-2 Standard, Sample Diluent, Anti-FITC Conjugated Detector Antibody, Concentrated Anti-FITC Peroxidase Conjugate, Conjugate Diluent, Substrate, Wash Buffer, Stop Solution, Adhesive Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
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<b>Hazardous Materials Attention:</b> Due to the nature of the Hazardous Materials in this shipment, additional shipping charges <i>may</i> be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
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| S Phrase |
S: 26-36/37/39-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 23/24/25-35-36/37/38-40-43 Causes severe burns. Limited evidence of a carcinogenic effect. May cause sensitization by skin contact. |
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| Data | |||
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 Neutralization of bcl-2 positive samples using a bcl-2 monoclonal antibody (Cat. No. OP60).  The pooled coefficient of variation (according to the formula of Henry, et al., 1974) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.  The pooled coefficient of variation (according to the formula of Henry, et al., 1975) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.  Human sera and plasma samples were spiked with bcl-2 protein to obtain a final concentration of 42 units/ml and analyzed as outlined above. The control represents bcl-2 spiked into sample diluent.  Dilution recovery from a bcl-2 positive cell line using different antigen extraction agents (different detergents).  Dilution recovery using different cell lines. |
