This kit allows the quantitation of apoptotic cells in vitro by DNA affinity-mediated capture of free nucleosomes followed by their anti-histone-facilitated detection. In this assay, mono- and oligonucleosomes are captured on pre-coated DNA binding proteins. Anti-Histone 3 (H3) biotin-labeled antibody then binds to the histone component of captured nucleosomes and is detected following incubation with streptavidin-HRP conjugate. HRP catalyzes the conversion of colorless tetramethylbenzidine to blue. The addition of stop solution changes the color to yellow, the intensity of which is proportional to the number of nucleosomes in the sample. By comparing the absorbance of unknown with that obtained from the standards, one can assign a nucleosome unit value to the unknown sample. Can be used to assign apoptotic index.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | a broad range of species | ||
| Applications |
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| Protect from Moisture | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit (U) is defined as the amount of nucleosomes from 444 UV-treated Daudi cells. | ||
| Sensitivity | 0.15 U/ml | ||
| Assay range | 00 - 12 U/ml | ||
| Assay time | 5 h | ||
| Sample type | Cell lysates | ||
| Kit contains | 96-Well Plate (12 removable strips) Coated with a DNA Binding Protein, Nucleosome Standard, Lysis Buffer/Sample Diluent, Biotinylated Anti-Histone 3 Antibody, 400X HRP-Streptavidin Conjugate, Conjugate Diluent, Chromogenic Substrate, 20X Concentrated Wash Solution, Stop Solution, 4 Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
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<b>Hazardous Materials Attention:</b> Due to the nature of the Hazardous Materials in this shipment, additional shipping charges <i>may</i> be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
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| S Phrase |
S: 26-36/37/39-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 23/24/25-35-36/37/38-40-43 Causes severe burns. Limited evidence of a carcinogenic effect. May cause sensitization by skin contact. |
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