ST1007
PhosphoDetect™ Anti-EGFR (pTyr99²) Rabbit pAb
Anti-Epidermal Growth Factor Receptor, Phospho-Specific (Tyr99²)
Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr992 in EGF-treated A431 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide surrounding the Tyr99² phosphorylation site of human EGFR | ||
| Form | Liquid | ||
| Formulation | In phosphate buffer, 0.1% BSA, pH 7.4. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | EGF treated A431 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of mouse epidermal growth factor receptor, phosphorylated at Tyr <sup>992</sup>, by immunoblotting. Samples: Whole cell lysate from serum-starved (30 h) NIH3T3 cells stimulated with EGF (30 ng/ml, 10 min; lanes 2, 4, 6) or left unstimulated (lanes 1, 3, 5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr<sup>992</sup>) Rabbit pAb (Cat. No. ST1007) pre-incubated without peptide (lanes 1, 2), with phosphopeptide immunogen (lanes 3, 4), or with non-phosphopeptide corresponding to the phosphopeptide immunogen (lanes 5, 6) (0.5 µg/ml). Detection: chemiluminescence. |
