Recognizes the ~51 kDa GSK-3α protein phosphorylated at Tyr279 and the ~47 kDa protein GSK-3β phosphorylated at Tyr216.
Note: 1T = 1 test. Sufficient for 10 Western miniblots.
| Accessories for PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb |
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Product information
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Host
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Rabbit
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Isotype
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IgG
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Immunogen
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a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3α/β
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Form
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Liquid
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Formulation
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In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
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Species reactivity
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human, mouse, rat
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Preservative
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≤0.1% sodium azide
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Applications
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Enzyme-Linked Immunosorbent Assay
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Dot Blot
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Immunocytochemistry
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Immunoblotting (Western Blotting)
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Avoid freeze/thaw
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Yes
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Positive control
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Serum starved insulin-treated 3T3-L1 cells
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Negative control
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Normal rabbit serum
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Store and ship information
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Storage
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-20°C
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Ship
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Blue or Dry Ice
Standard Handling
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Canadian export regulations
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Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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Data
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Detection of human GSK-3β, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3α/β (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
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