70663
Proteinase K, Lyophilized
Efficient removal of proteins from nucleic acid solutions
For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
09 February 2012
loading
Proteinase K is a highly active 28,904-Da
serine protease isolated from the fungus
Tritirachium album. The enzyme exhibits
broad cleavage specificity on native and
denatured proteins and is widely used in the
purification of DNA and RNA. Its activity
is increased in the presence of denaturants
such as SDS (1%) and elevated temperature
(50-60°C). The recommended working
concentration is 50-100 µg/ml for protein
removal and enzyme inactivation, and up to
2 mg/ml for tissue treatment. The Proteinase K,
Lyophilized powder can be prepared as a
20 mg/ml stock solution in water and stored
in aliquots at -20°C. The enzyme is also
available as a ready-to-use concentrated stock
solution (600 mAU/ml) that is convenient
for routine use in most applications. 1 mg
of Proteinase K is the equivalent of 30 mAU
(AU = Anson unit). The Novagen Proteinase
K products are free of detectable DNase and
RNase
| Product information | |||
|---|---|---|---|
| Unit definition | One AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C. | ||
| CAS number | 39450-01-6 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Harmful |
||
| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 22-24-26-36/37 Do not breathe dust. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves. |
||
| R Phrase |
R: 36/37/38-42 Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation. |
||
