RiboJuice™ siRNA Transfection Reagent

RiboJuice

siRNA Transfection Reagent for mammalian cells

RiboJuice™ siRNA Transfection Reagent efficiently delivers small interfering RNA (siRNA) into a wide range of mammalian cell lines for targeted gene suppression. When transfected into the cell, the siRNA directs cleavage of the target message resulting in suppression of protein expression without affecting non-target genes. RiboJuice is effective for siRNA delivery in a variety of cell lines with multiple siRNAs Figure 1. Concentration dependence and specificity of siRNA(b-gal)-mediated suppression and Figure 2. Suppression of b-galactosidase expression in various cell lines). The ability to target specific genes for gene silencing is a powerful tool that researchers can now perform more easily, using RiboJuice siRNA Transfection Reagent.

Figure 1. Concentration dependence and specificity of siRNA(b-galactosidase)-mediated suppression

CHO-K1 and HEK 293 cells plated at 50,000 cells per well were transfected after 24 h with two mixtures. One mixture contained 1 µl GeneJuice™ Transfection Reagent, 0.25 µg pTriEx™-2(b-gal), and 0.025 µg pTriEx-2(Fluc). The other mixture contained 3 µl RiboJuice™ siRNA Transfection Reagentand either 0.1, 0.25, 0.5, 1, 2.5, 5, 10, or 25 nM (final concentration) of the indicated siRNAs. As a control, 3 µl RiboJuice was also used without any siRNA. Total volume per well was 300 µl. Cells were lysed with Reportasol™ Extraction Buffer 24 h after transfection and assayed for reporter activity. All assays were performed in triplicate and variation is expressed as standard error of the mean.

Figure 2. Suppression of b-galactosidase expression in various cell lines

The indicated cell lines were plated at 50,000 cells per well and transfected after 24 h with two mixtures. One mixture contained 1 µl GeneJuice Transfection Reagent, 0.25 µg pTriEx-2(b-gal), and 0.025 µg pTriEx™-2(Rluc). Mixture two contained 3 µl RiboJuice siRNA Transfection Reagent and 10 nM (final concentration) siRNA(b-gal)1. For each cell line, a reaction lacking siRNA was used as a control (normalized to 100%). Total volume per well was 300 µl. Cells were lysed with Reportasol™ Extraction Buffer24 h after transfection and assayed for reporter activity. All assays were performed in triplicate and variation is expressed as standard error of the mean.

For more information on RiboJuice see inNovations 14.