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Fractoprep® Biochromatography Media
| Cat. No. |
Description |
Contents
[ml] |
Particle Size
[µm] |
Capacity
[per ml gel] |
| 1.17973 |
Strong anion exchanger Fractoprep® TMAE |
100, 500, 1000, 5000 |
30-50 µm |
100mg BSA |
| 1.17971 |
Weak anion exchanger Fractoprep® DEAE |
100, 500, 1000, 5000 |
30-50 µm |
100mg BSA |
| 1.17972 |
Cation exchanger Fractoprep® SO3 |
100, 500, 1000, 5000 |
30-50 µm |
100mg lysozyme |
The Fractoprep line of biochromatography media utilizes the
same effective tentacle-based chemistry found in the Fractogel EMD
product line except in Fractoprep, functionally-substituted acrylamides
are grafted onto porous particles of hydrophilic vinyl polymer resin.
The resulting ion exchange media have excellent protein binding capacities
at high flow rates, with enhanced mechanical and chemical stability.
The resins are extremely stable against sodium hydroxide treatment, an
important clean-in-place procedure commonly used in biopharmaceutical
manufacturing. Furthermore, they are mechanically rigid due to the high
level of cross-linking in the matrix polymer which makes Fractoprep very easy
to handle and pack for developmental and commercial-scale manufacturing applications.
Use Fractoprep for:
| • |
efficient purification of biological molecules
such as antibodies, blood plasma factors, recombinant proteins |
| • |
DNA removal |
| • |
endotoxin removal |
| • |
virus reduction/purification |
Benefits
| Features |
Advantages |
| High capacity at high flow rates |
High throughput |
| High pressure stability |
High flow rates |
| High chemical stability |
Easy clean-in-pace |
| Strong binding of proteins |
Efficient capture |
| High recovery of biological activities |
High yield |
| Efficient removal of viruses, DNA and endotoxins |
High purity of the target molecule |
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Chemistry
Fractoprep® resins consist of the following components:
| • |
Bead polymer - hydrophilic vinyl polymer resin |
| • |
Tentacle - acrylamide polymers |
| • |
Functions groups – TMAE, DEAE and SO3 |
The Fractoprep® line of media consists of two anion-exchange resins
Fractoprep® TMAE and Fractoprep® DEAE and a
strong cation-exchange resin Fractoprep® SO3. The beads have a particle size
that range from 30 to 150 µm and consist of large pores that enable free diffusion of proteins into the
beads allowing for high throughput at very low back pressures.
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Fractoprep resins consist of the following components:
• Bead polymer - hydrophilic vinyl polymer resin
• Tentacle - acrylamide polymers
• Functions groups - TMAE, DEAE and SO3
The Fractoprep line of media consists of two anion-exchange resins Fractoprep TMAE and
Fractoprep DEAE and a strong cation-exchange resin Factorprep SO3. The beads
have a particle size that range from 30 to 150 µm an consist of large pores that enable
free diffusion of proteins into the beads allowing for high throughput at very low back pressures.
For more information, please download...
Biochromatography Media Overview
Fractoprep Biochromatography Media
Antibody Purification with EMD Biochromatography Media
Using EMD Ion Exchange Biochromatography Media |
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