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Self-Packer® Packing Stands

Product Listing

Until recently, only ready packed columns were available for preparative use in the chromatography laboratory. These relatively expensive columns were available with a limited selection of stationary phases so they were not suitable for the whole application range of preparative chromatography.

Even conventional column packing systems, e.g. high-pressure autoclaves and similar filling techniques, were not fully satisfactory as the quality of such self-packed columns was rarely adequate.

In co-operation with our in-house customers (pharmaceutical research, process development, etc.), we have developed a completely new column packing technology:

Packing Stands for Preparative Chromatography in the Laboratory, Pilot Plant and Production

These packing stands combine components from the various developments the column program from Merck KGaA, Darmstadt, Germany, including the patented A/E column technology developed by our subsidiary company Separations Technology, USA.

Packing stands are available with internal diameters 25mm, 50mm and 100mm for laboratory and pilot plant use.

Packing stands of different dimensions can of course be made available for special applications upon request.

Such special versions create no problems for us; just contact us, we will be glad to help.

The basic principle associated with column filling is the application of a simple vacuum technology combined with column bed compression for stabilising the sorbent packing.

Intensive studies have shown that it is very important to avoid the formation of bridges between the sorbent particles during the packing process.

In contrast to the conventional method of rapid filtration under pressure and with a high linear flow rate (or the compression of viscous particle suspensions in the column bed), the application of a normal water jet vacuum provides very good results, especially with modern high performance stationary phases.

This particular column filling procedure enables exact dosing of the packing quantity or the packing bed height and is therefore suitable for studies for determining the optimal column geometry in production procedures. The precisely settable filling quantities enable direct correlation between maximum loading capacity and up-scaling to larger column dimensions. Using the example of a packing stand with 50 mm internal diameter, the simple filling procedure will be explained:

  • Assembly with the help of detailed instructions.

  • Manufacture of the stationary phase suspension (e.g. 200g) for RP-phases with acetone, or for silica phases with methanol.

  • Degassing briefly in an ultrasonic bath (3-5 minutes).

  • Filling the suspension and application of a normal water jet vacuum.

  • Aspiration of the suspension (duration dependent on the particle size).

  • Closing top flange the column and hydraulic compression of the packing.

  • Connection to the pump and short application of maximum flow rate to the packing.

  • Subsequent compression of the packing until stable (2-3 times).

  • Testing the column quality using a test mixture.

  • The column is now ready for use in production.

Advantages:

The new vacuum filling technology is simple to use for preparative columns.

The columns can be rapidly and economically filled wherever required.

Due to the controlled bed compression, column usage time and lifetime of the column are prolonged.

"Soft" gel such as polymer gels can also be used without problems.

Due to the stabilized column bed in conjunction with compression technology, the packed columns can also be used outside of the hydraulic unit.



Packing columns with the new packing stands of 2

Materials Components
Stainless Steel 1.4571 Column tubing and all steel elements
PTFE (polytetrafluoroethylene) Seals (including capillaries for NW100)
Stainless Steel 1.4404 Frits
PEEK (polyether-etherketone) Capillaries (except NW100)
PVDF (polyvinylidenedifluoride) Frit holder


Technical Data for Packing Stands
  Packing Stand NW25 Packing Stand NW50 Packing Stand NW100
Max. packing height (mm) 220 220 350
Min. packing height (mm) 170 170 300
Max. working pressure (bar) 200 100 100
Weight (kg) approx. 20 approx. 22 approx. 70

The 50 and 100mm i.d. filling stands can be ordered either with the economic top screw flange or with the more comfortable chain. The 100 mm packing stand with the bolted top flange is very comfortable when stationary phases have to be replaced frequently.

APPLICATION AND TECHNICAL DATA SUPPLIED BY EMD CHEMICALS IS BELIEVED TO BE RELIABLE BUT IS OFFERED FOR INFORMATION PURPOSES ONLY AND AS A GUIDE FOR PROPERLY TRAINED PERSONS HAVING THE NECESSARY SKILLS.


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EMD Chemicals is Merck KGaA, Darmstadt, Germany, in the US and Canada.